BIOSPYDER TECHNOLOGIES, INC.

AH52-NATURAL RESOURCES AND ENVIRONMENT R&D SERVICES.

AH52 - NATURAL RESOURCES AND ENVIRONMENT R&D SERVICES.

AH91 - HIGH THROUGHPUT TRANSCRIPTOMICS (HTTR)

AH52 - NATURAL RESOURCES AND ENVIRONMENT R&D SERVICES

CONTRACT TO PERFORM SINGLE-CELL TRANSCRIPTOMIC ANALYSIS OF ENVIRONMENTAL CHEMICAL-INDUCED EFFECTS ON 48 SAMPLES USING THE TEMPO-LINC TECHNOLOGY. IPP APPROVER: SANDRA ROBERTS ALTERNATE IPP APPROVER: DANIEL HALLINGER

AH52 - NATURAL RESOURCES AND ENVIRONMENT R&D SERVICES - A TOTAL OF 2016 TISSUE SAMPLES WILL BE SUBMITTED TO BIOSPYDER FOR MULTIPURPOSE-RNA ISOLATION AND TEMPO-SEQ ANALYSIS. EACH SAMPLE WILL BE SEQUENCED TO A MINIMUM MAPPED READ PER SAMPLE OF 50 M

AH52 - NATURAL RESOURCES AND ENVIRONMENT R&D SERVICES - ENAP PROOF-OF-CONCEPT STUDY. PERFORM TARGETED RNA-SEQ (I.E. TEMPO-SEQ) ANALYSIS OF CELL LYSATES GENERATED AT THE USEPA CCTE LABORATORY. THE SAMPLES WILL BE SUPPLIED TO BIOSPYDER IN A SERIES

TO TITLE: GLTED HIGH THROUGHPUT TARGETED TRANSCRIPTOMICS. TARGETED RNA-SEQ (TEMPOSEQ) ANALYSES OF WHOLE BODY HOMOGENATE OR PURIFIED RNA SAMPLES AT 97-5000 SAMPLE/TASK ORDER SCALE.

HIGH-THROUGHPUT TRANSCRIPTOMICS (HTTR). ORDER FOR 2,922 SAMPLES AT A 3M READ DEPTH ON THE BIOSPYDER IDIQ CONTRACT 68HERC20D0028, INTERNAL TASK ORDER 52 (NCCRP PROJECT/OPPT) FOR TRACKING PURPOSES WITH A 1 YEAR PERIOD OF PERFORMANCE. SEE ATTACHED ORDER

BIOSPYDER IDIQ CONTRACT 68HERC20D0028. 510 SAMPLES SEQUENCED TO A MINIMUM MAPPED READ PER SAMPLE OF 3 MILLION USING THE TEMPO-SEQ HUMAN WHOLE TRANSCRIPTOME ASSAY.

BIOSPYDER IDIQ CONTRACT 68HERC20D0028. 510 SAMPLES SEQUENCED TO A MINIMUM MAPPED READ PER SAMPLE OF 3 MILLION USING THE TEMPO-SEQ HUMAN WHOLE TRANSCRIPTOME ASSAY. POP 01/09/2025 - 01/08/2026

BIOSPYDER IDIQ CONTRACT 68HERC20D0028, INTERNAL TASK ORDER 53 FOR TRACKING PURPOSES WITH A 1 YEAR PERIOD OF PERFORMANCE. THE 271 SAMPLES WILL BE SUPPLIED TO BIOSPYDER FOR TEMP-SEQ ANALYSIS. SEE ATTACHED ORDER SHEET FOR SPECIFICS. CO: MARK CRANLEY

2426D3C406 BIOSPYDER IDIQ CONTRACT 68HERC20D0028. INTERNAL TASK ORDER 51 (CPHEA-MURRAY) FOR TRACKING PURPOSES. THREE PHASE PROJECT WITH EACH SAMPLE SEQUENCED TO A MINIMUM MAPPED READ PER SAMPLE OF 3 MILLION USING THE TEMPO-SEQ HUMAN WHOLE TRANSCRIPTO

2426D3C007 BIOSPYDER IDIQ CONTRACT 68HERC20D0028. INTERNAL TASK ORDER 49 (PHITD) FOR TRACKING PURPOSES WITH A 1 YEAR PERIOD OF PERFORMANCE. A TOTAL OF 1410 SAMPLES WILL BE SUBMITTED TO BIOSPYDER FOR TEMPO-SEQ ANALYSIS. SEE ATTACHED ORDER SHEET

NEW TO (INTERNAL NUMBER 50) FOR BIOSPYDER CONTRACT 68HERC20D0028 ETAP -MULTIPURPOSE-RNA ISOLATION AND TEMPO-SEQ ANALYSIS.

A TOTAL OF 80 SAMPLES WILL BE SUBMITTED TO BIOSPYDER FOR TEMPO-SEQ ANALYSIS. THE SAMPLES WILL BE SUPPLIED TO BIOSPYDER IN A SERIES OF MICROFUGE TUBES WITH ONE SAMPLE PER TUBE. EACH SAMPLE WILL BE SEQUENCED TO A MINIMUM MAPPED READS PER SAMPLE OF 1.07

THE PURPOSE OF THIS MODIFICATION IS TO CHANGE THE POP END DATE FROM 06/22/2024 TO 09/22/2024 TO ADD 3 MONTHS FOR RE-RUN OF ADDITIONAL 2922 SAMPLES AT THE COST OF YEAR 3 PRICING. ALL OTHER TERMS AND CONDITIONS REMAIN UNCHANGED. CO/CS -MARK CRANLEY /

BIOSPYDER TO INTERNAL TASK #47 A TOTAL OF 96 SAMPLES WILL BE SUBMITTED TO BIOSPYDER FOR TEMPO-SEQ ANALYSIS. THE SAMPLES WILL BE SUPPLIED TO BIOSPYDER IN A SERIES OF BARCODED 96-WELL MICROTITER PLATES WITH ONE SAMPLE PER WELL. EACH SAMPLE WILL BE SE

INTERNAL BIOSPYDER TO #46 A TOTAL OF 444 LYSATE SAMPLES WILL BE SUBMITTED TO BIOSPYDER FOR TEMPO-SEQ ANALYSIS. THE SAMPLES WILL BE SUPPLIED TO BIOSPYDER IN A SERIES OF BARCODED 384-WELL MICROTITER PLATE WITH ONE SAMPLE PER WELL. EACH SAMPLE WILL BE S

CCTE-GLTED-STB - EDNA TEMPO-SEQ PLATFORM

A TOTAL OF 1008 TISSUE SAMPLES WILL BE SUBMITTED TO BIOSPYDER FOR MULTIPURPOSE-RNA ISOLATION AND TEMPO-SEQ ANALYSIS. IPP APPROVER: SANDRA ROBERTS ALTERNATE APPROVER: DAVID MURPHY

FUND EXERCISE OF OPTION MOD.

A TOTAL OF 192 SAMPLES WILL BE SUBMITTED TO BIOSPYDER FOR TEMPO-SEQ ANALYSIS. EACH SAMPLE WILL BE SEQUENCED TO A MINIMUM MAPPED READS PER SAMPLE OF 4.5 MILLION USING THE TEMPO-SEQ HUMAN WHOLE TRANSCRIPTOME ASSAY. . BIOSPYDER WILL CONSULT WITH THE CCT

A TOTAL OF 96 SAMPLES WILL BE SUBMITTED TO BIOSPYDER FOR TEMPO-SEQ ANALYSIS. THE SAMPLES WILL BE SUPPLIED TO BIOSPYDER IN A SERIES OF BARCODED 96-WELL MICROTITER PLATES WITH ONE SAMPLE PER WELL.

EXERCISE OY#1: TRANSCRIPTOME SEQUENCING SERVICES - MOUSE SURROGATE S1500+ TEMPO-SEQ KIT (INCLUDING DOS, TEMPO-SEQ REAGENTS, AND STANDARD ATTENUATION), TEMPO-SEQR PROJECT ACCESS, AND HISEQ 2500 SEQUENCING RUN.

FUND EXERCISE OF OPTION MOD.

TARGETED RNA-SEQ (TEMPOSEQ) ANALYSES OF WHOLE BODY HOMOGENATE OR PURIFIED RNA SAMPLES AT 97-5000 SAMPLE/TASK ORDER SCALE AND RNASEQ EQUIVALENT SPECIFICATION OF APPROXIMATELY 30 MILLION MAPPED READS PER SAMPLE.

BIOSPYDER 68HERC20D0028, INTERNAL TO40 FOR 4536 CELL LYSATE SAMPLES. EACH SAMPLE WILL BE SEQUENCED TO A MINIMUM MAPPED READ PER SAMPLE OF 3 MILLION USING THE TEMPO-SEQ HUMAN WHOLE TRANSCRIPTOME ASSAY. SEE ATTACHED ORDER SHEET FOR SPECIFICS. SEE ATTAC

BIOSPYDER 68HERC20D0028, INTERNAL TO42 FOR 15,492 CELL LYSATE SAMPLES WHICH WILL BE SEQUENCED TO A MINIMUM MAPPED READ PER SAMPLE OF 3 MILLION USING THE TEMPO-SEQ HUMAN WHOLE TRANSCRIPTOME ASSAY.

BIOSPYDER 68HERC20D0028, INTERNAL TO41 FOR 2268 CELL LYSATE SAMPLES. EACH SAMPLE WILL BE SEQUENCED TO A MINIMUM MAPPED READ PER SAMPLE OF 4.5 MILLION USING THE TEMPO-SEQ HUMAN WHOLE TRANSCRIPTOME ASSAY. SEE ATTACHED ORDER SHEET FOR SPECIFICS.

192 CELL LYSATE SAMPLES, EACH SAMPLE WILL BE SEQUENCED TO A MINIMUM MAPPED READ PER SAMPLE OF 3 MILLION USING THE TEMPO-SEQ HUMAN WHOLE TRANSCRIPTOME ASSAY. BIOSPYDER 68HERC20D0028. TOCOR: SANDRA ROBERTS CO: MARK CRANLEY

BIOSPYDER IDIQ CONTRACT 68HERC20D0028. INTERNAL TASK ORDER 38 (CORTON) FOR TRACKING PURPOSES WITH A 1 YEAR PERIOD OF PERFORMANCE. A TOTAL OF 768 SAMPLES WILL BE SUBMITTED TO BIOSPYDER FOR TEMPO-SEQ ANALYSIS.

BIOSPYDER IDIQ CONTRACT 68HERC20D0028. NEW TO FOR TRACKING PURPOSES WITH A 1 YEAR PERIOD OF PERFORMANCE. A TOTAL OF 192 SAMPLES WILL BE SUBMITTED TO BIOSPYDER FOR TEMPO-SEQ ANALYSIS.

DETERMINE CHANGES IN GENE EXPRESSION IN RAT PRIMARY HEPATOCYTES AFTER EXPOSURE TO CHEMICALS AS PART OF THE ONGOING RESEARCH IN SUPPORT OF THE CHEMICAL SAFETY FOR SUSTAINABILITY (CSS) STRATEGIC RESEARCH ACTION PLAN.

TECHNICAL DIRECTION FOR BIOSPYDER TO PERFORM TARGETED RNA-SEQ (I.E. TEMPO-SEQ) ANALYSIS OF CELL LYSATES GENERATED AT THE CCTE LABORATORY. A TOTAL OF 378 SAMPLES WILL BE SUBMITTED TO BIOSPYDER FOR TEMPO-SEQ ANALYSIS. THE SAMPLES WILL BE SHIPPED TO BIO

INTERNAL TRACKING #34 BIOSPYDER-IDIQ 68HERC20D0028-IN SUPPORT OF CCTE/OPP/NCCRP WORK. PERFORM TARGETED RNA-SEQ (I.E. TEMPO-SEQ) ANALYSIS OF CELL LYSATES GENERATED AT THE USEPA CCTE LABORATORY. THE RESULTING DATA WILL BE USED BY CCTE SCIENTISTS TO DET

BIOSPYDER IDIQ 68HERC20D0028. NEW TASK ORDER. BIOSPYDER TO PERFORM TARGETED RNA-SEQ (I.E. TEMPO-SEQ) ANALYSIS OF CELL LYSATES AND RODENT TISSUE SAMPLES GENERATED AT THE USEPA CCTE LABORATORY. THE RESULTING DATA WILL BE USED BY CCTE SCIENTISTS TO DET

NEW TO FOR BIOSPYDER 68HERC20D0028 INTERNAL TO 32, TO: 68HERC23F0121. CCED LEAH WEHMAS REQUEST. TECHNICAL DIRECTION FOR BIOSPYDER TO PERFORM RNA PURIFICATION AND TARGETED RNA-SEQ (IE. TEMPO-SEQ) ANALYSIS ON KIDNEYS, LIVERS, BRAINS, TESTES (MALES), OV

EXERCISE OY#1: TRANSCRIPTOME SEQUENCING SERVICES - MOUSE SURROGATE S1500+ TEMPO-SEQ KIT (INCLUDING DOS, TEMPO-SEQ REAGENTS, AND STANDARD ATTENUATION), TEMPO-SEQR PROJECT ACCESS, AND HISEQ 2500 SEQUENCING RUN.

BIOSPYDER IDIQ CONTRACT 68HERC20D0028. INTERNAL TASK ORDER 30 FOR TRACKING PURPOSES WITH A 1 YEAR PERIOD OF PERFORMANCE. A TOTAL OF 192 SAMPLES WILL BE SUBMITTED TO BIOSPYDER FOR TEMPO-SEQ ANALYSIS TO DETERMINE CHANGES IN GENE EXPRESSION IN THE TISSU

BIOSPYDER IDIQ CONTRACT 68HERC20D0028. INTERNAL TASK ORDER 31 FOR TRACKING PURPOSES WITH A 1 YEAR PERIOD OF PERFORMANCE. A TOTAL OF 768 SAMPLES WILL BE SUBMITTED TO BIOSPYDER FOR TEMPO-SEQ ANALYSIS TO DETERMINE CHANGES IN GENE EXPRESSION IN RAT PRIMA

BIOSPYDER IDIQ CONTRACT 68HERC20D0028. INTERNAL TASK ORDER 29 FOR TRACKING PURPOSES WITH A 1 YEAR PERIOD OF PERFORMANCE. A TOTAL OF 768 SAMPLES WILL BE SUBMITTED TO BIOSPYDER FOR TEMPO-SEQ ANALYSIS TO DETERMINE THE EFFECTS OF PRODUCED WATER ON GENE E

BIOSPYDER IDIQ CONTRACT 68HERC20D0028 NEW TO (27). INTERNAL TASK ORDER 26 FOR TRACKING PURPOSES WITH A 1 YEAR PERIOD OF PERFORMANCE. A TOTAL OF 24,948 SAMPLES WILL BE SUBMITTED TO BIOSPYDER FOR TEMPO-SEQ ANALYSIS - HBEC3-KT_GAP_FILL. CONTRACT SP

NEW TASK ORDER REQUEST UNDER BIOSPYDER 68HERC20D0028/INTERNAL TRACKING NUMBER 25 TO COMPLETE WORK ON ORIGINAL INTERNAL TASK #14 SEE ATTACHED MEMO REGARDING DESCOPE AND DEOBLIGATION FROM 68HERC21F0334 AND REOBLIGATE $10K TO THE NEW TASK ORDER CS PRA

PR-ORD-22-02316: 2226B3E090: ITEM #1-TARGETED RNA-SEQ (TEMPOSEQ) ANALYSES OF WHOLE BODY HOMOGENATE OR PURIFIED RNA SAMPLES AT 97-5000 SAMPLE/TASK ORDER SCALE AND RNASEQ EQUIVALENT SPECIFICATION OF 30 MILLION MAPPED READS PER SAMPLE, 385-3000 GENE ASS

BIOSPYDER IDIQ CONTRACT 68HERC20D0028, INTERNAL TASK ORDER 14 CORTON'S LAB 384 SAMPLES IN A BARCODED 384-WELL MICROTITER PLATE WITH ONE SAMPLE PER WELL. SEE ATTACHED ORDER SHEET.

PR-ORD-22-02134: 2226B2E034: NEW TO FOR BIOSPYDER 68HERC20D0028 INTERNAL TO 24: 68HERC22F0495; FUND WITH PFAS 1.6.9 POP 12 MONTHS FROM DATE OF AWARD CS: PRADEEP KANDAMBATH CO: MARK CRANLEY COR: SANDRA ROBERTS

BIOSPYDER IDIQ CONTRACT 68HERC20D0028 NEW TO (26). INTERNAL TASK ORDER 26 FOR TRACKING PURPOSES WITH A 1 YEAR PERIOD OF PERFORMANCE. A TOTAL OF 30 SAMPLES WILL BE SUBMITTED TO BIOSPYDER FOR TEMPO-SEQ ANALYSIS. CONTRACT SPECIALIST: PRADEEP KANDAMB

2226D3C405 BIOSPYDER IDIQ CONTRACT 68HERC20D0028 NEW TO (28). INTERNAL TASK ORDER 28 FOR TRACKING PURPOSES WITH A 1 YEAR PERIOD OF PERFORMANCE. A TOTAL OF 58 SAMPLES WILL BE SUBMITTED TO BIOSPYDER FOR TEMPO-SEQ ANALYSIS. CONTRACT SPECIALIST: PRADE

THE CONTRACTOR IS RESPONSIBLE FOR PROVIDING SUPPORT TO EVALUATE TREATMENT-RELATED CHANGES IN GENE EXPRESSION IN SAMPLES COLLECTED FROM ANIMAL OR PLANT SPECIES OF INTEREST TO EPA. EXAMPLES OF AVAILABLE TECHNOLOGIES THAT CAN PROVIDE THESE TYPES OF DATA